Antibacterial Effect of Silver Nanoparticles Prepared Using Pterospermum Diversifolium Semi-Solid Extract

Abstract

A green technique was used to create silver nanoparticles (AgNPs) using Pterospermum diversifolium semi-solid extract. The leaves were processed into a soft extract to retain the active ingredients of the leaves for a long time. The extract acts as a reducing agent to form Ag atoms and as a surfactant to create a stable environment for AgNPs. Spherical silver nanoparticles with a diameter of about 20 - 50 nm ± 5 nm were formed and were well stable in the solution. Optical properties, as well as structure, size, size distribution, and composition of elements and functional groups of AgNPs, were investigated through absorbance spectra, transmission electron microscopy (TEM), dynamic light scattering (DLS), Energy-dispersive X-ray spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) measurements. The extract and AgNPs were used to study the antibacterial effect on bacterial strains Ecoli, P. aeruginosa, and S. Aureus, with the positive control being Amoxicillin. The results showed that AgNPs and leaf extract with a 100 µg/mL concentration have better antibacterial ability than antibiotic amoxicillin 50 µg/mL.

Received:  24 February 2024 | Revised: 17 July 2024 | Accepted: 12 October 2024

Conflicts of Interest

The authors declare that they have no conflicts of interest to this work.

Data Availability Statement

Data sharing is not applicable to this article as no new data were created or analyzed in this study.

Author Contribution Statement

Hue Thi Do: Conceptualization, Software, Validation, Formal analysis, Resources, Writing - original draft, Writing - review & editing, Visualization, Supervision, Project administration. Trung Anh Le: Methodology, Formal analysis, Investigation, Data curation, Writing - review & editing.